Coding
nuclease_y
Part:BBa_K3027002:Design
Designed by: Arnaud Boudigou Group: iGEM19_GO_Paris-Saclay (2019-09-24)
Nuclease_yqcG CDS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1235
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Modification for compatibility with type IIs cloning (Golden Gate)
To make the biobrick compatible with type IIs cloning, a BsaI site has been “erased” by replacing a Cytidine with a Guanosine at 804 position. This substitution is synonymous as the codons GTC and GTG both encode a Valine in position 268 of the protein.
Source
From Bacillus subtilis